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. 2009 May 15;20(10):2522–2529. doi: 10.1091/mbc.E08-07-0772

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© 2009 by The American Society for Cell Biology

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Figure 3. — Sec10 knockdown prevents MDCK cystogenesis. A) Control, Sec10-overexpressing (OE), and Sec10 knockdown (KD) MDCK cells were seeded at low (single-cell) density in a collagen matrix. By 8 d, the Sec10 OE cells had already formed mature cysts, with a single lumen. Imaging of live cysts was done using Nomarski optics. (B) At 12 d, fixation and immunofluorescence staining of actin with phalloidin-rhodamine, showed maturing cysts in the control cells, mature cysts in the Sec10 OE cells, and immature cysts or clumps of cells in the Sec10 KD cells. Bar, 20 μm. Fully mature control cysts are seen in the majority of cases by 15 d (see Figure 4C).