Figure 5.
Contribution of single amino acid residues to the signal. (A) After import of 35S-labeled Tim9 and Tim9L31A,V32A precursors, mitochondria were subjected to native analysis or were treated with Prot. K and analyzed by reducing SDS electrophoresis. (B) Assembly of Tim10 and mutant Tim10L36A,V37A in wild-type mitochondria was analyzed by native electrophoresis. (C) Sequence similarity of the Tim9 signal among eukaryotic species. (D and E) Various Tim9 precursors were imported into wild-type mitochondria for the indicated times. Samples were treated by proteinase K (D) or applied directly (E) to nonreducing SDS electrophoresis. Asterisk, nonspecific band.