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. 2009 May 15;20(10):2563–2571. doi: 10.1091/mbc.E08-10-1019

Figure 2.

Figure 2.

Microscopy analysis of shelterin components in Trf1-deficient cells. (A) Cells of the indicated genotype were fixed and subjected to in situ hybridization with a PNA anti-telomere probe (red) then stained with antibodies to Trf2 (green) and counterstained with DAPI (blue). Cells of the indicated genotype were transiently transfected with expression vectors for myc-Rap1 (B) or myc-Pot1 (C) and then fixed and stained with antibodies to myc (red) and to Trf2 (green) and counterstained with DAPI (blue). Scale bars, 10 μm.