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. 2009 May 15;20(10):2593–2604. doi: 10.1091/mbc.E08-07-0790

Figure 2.

Figure 2.

Intracellular Cx43 localized predominantly to the Golgi apparatus in ERp29-depleted cells. ROS cells were transfected with either ERp29 siRNA (A–I) or with control siRNA (J–R) and further incubated for 48 h. The cells were then fixed and immunostained for Cx43 (A, D, G, J, M, and P) and an ER marker (PDI) (B and K), cis-Golgi marker (GM130) (E and K), or early endosome-associated protein 1 (EEA1) (H and Q). Merged images are in C, F, I, L, O, and R. In cells treated with ERp29 siRNA, Cx43 was retained in an intracellular compartment (arrowheads) that colocalized predominantly with GM130 (G–I). To a lesser extent, Cx43 also colocalized with PDI, in cells treated with ERp29 siRNA. (C) Colocalization between Cx43 and EEA1 was limited (I). Cells treated with control siRNA showed less intracellular Cx43. Bar, 10 μm.