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. 2009 May 15;20(10):2615–2625. doi: 10.1091/mbc.E08-12-1162

Figure 1.

Figure 1.

Both domains of Mrpl36 are critical for respiratory growth. (A) Schematic representation of Mrpl36 and mrpl36ΔC. The predicted probabilities to form coiled-coil structures (Lupas, 1997) are expressed in percentage. MTS, mitochondrial targeting signal. (B) Deletion of the CE domain of Mrpl36 reduces growth on nonfermentable carbon sources. The indicated cells were grown in full medium containing galactose to log phase. Serial 10-fold dilutions were spotted on YP plates containing 2% glucose or 2% glycerol, and plates were incubated at 30°C for 2 and 4 d, respectively. (C) The L31 domain is sufficient to support respiratory growth. The indicated strains were grown on synthetic medium containing galactose to log phase. Serial 10-fold dilutions were spotted on synthetic media containing 2% glucose or 2% glycerol, and plates were incubated at 30°C for 3 and 6 d, respectively. (D) Steady-state levels of the different Mrpl36 forms. Lysates of the indicated cells grown on galactose-containing media were prepared and analyzed by Western blotting using antibodies against Tom70 (loading control), the HA-tag, and Mrpl36. Black arrow indicates Mrpl36ΔC, and white arrow indicates Mrpl36ΔC-HA. Asterisk (*), possible degradation product.