Figure 5.
Interaction of ribosomal subunits is destabilized in mrpl36ΔC mitochondria. Translation products of wild-type (A) or mrpl36ΔC (B) mitochondria were labeled with [35S]methionine. (C) Labeling of translation products in wild-type mitochondria was stopped after 20 min by the addition of puromycin. Next, mitochondria were reisolated, lysed with 1% dodecyl-maltoside, and the cleared lysate subjected to velocity centrifugation on a linear sucrose gradient. The gradient was fractionated and analyzed by autoradiography and Western blotting. Quantifications of the signals for Mrp20 and Mrp51 are presented.