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. 2009 May 15;20(10):2626–2637. doi: 10.1091/mbc.E08-11-1150

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© 2009 by The American Society for Cell Biology

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Figure 1. — SAC activation restrains SPBs separation in cin8-3 kip1Δ mutants. Wild-type (ySP4935), cin8-3 kip1Δ (ySP5361), cin8-3 kip1Δ mad2Δ (ySP5364), cin8-3 kip1Δ mad3Δ (ySP5363), cin8-3 kip1Δ bub3-WDd1 (ySP5360), and cin8-3 kip1Δ CDC20-107 (ySP5366) cells were arrested in G1 with α-factor at 25°C and released at 34°C (time 0). (A and B) Samples were collected at the indicated times for fluorescence-activated cell sorting (FACS) analysis of DNA contents and to follow kinetics of budding, SPB separation and mitotic spindle formation and elongation. (C) Distribution of distances between SPBs. (D) Micrographs of SPBs were taken 90′ after release. Bar, 2 μm. (E) Examples of anaphase cells at 90′ after release after nuclear staining with propidium iodide. Bar, 2 μm.