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The SAC restrains SPB separation through inhibition of Cdc20/APC. (A) stu1-5 (ySP6892), stu1-5 mad2Δ (ySP6625), stu1-5 mad2Δ cdc20-3 (ySP6665), stu1-5 mad3Δ (ySP6891) stu1-5 mad3Δ cdc20-3 (ySP6789) cells and (B) stu1-5 (ySP6892), stu1-5 mad2Δ (ySP6625), stu1-5 mad2Δ cdc16-1 (ySP7211) mutants were arrested in G1 with α-factor at 25°C and released at 37°C. Distances between SPBs were measured 120′ after release.
