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Securin restrains SPB separation in stu1-5 cells. (A) stu1-5 (ySP6892) and stu1-5 pds1Δ (ySP7068) mutants were arrested in G1 with α-factor at 25°C and released at 37°C. Distances between SPBs were measured 150 min after release. (B) stu1-5 (ySP6892), stu1-5 mad2Δ (ySP6625) and stu1-5 mad2Δ GAL1-PDS1mdb (ySP7572) mutants were grown in YEPR, arrested in G1 with α-factor at 25°C, and then released in YEPRG medium at 37°C. Distances between SPBs were measured 135′ after release.
