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. 2009 Feb 19;82(3):542–549. doi: 10.1093/cvr/cvp059

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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: journals.permissions@oxfordjournals.org.

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that the original authorship is properly and fully attributed; the Journal, Learned Society and Oxford University Press are attributed as the original place of publication with correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org.

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Leoligin inhibits the proliferation of smooth muscle cells (SMCs) without causing cell death. In order to investigate the effect of leoligin on the cellular level, isolated primary human vascular SMCs were incubated with the indicated concentrations of leoligin or solvent control (DMSO) for the indicated times. The right diagram shows a FACS analysis of cell viability determined by annexin V/propidium iodide staining. Values are means of a representative experiment performed in triplicate. The experiment was repeated three times, with similar results. The left diagram shows a cell proliferation analysis by the XTT assay. Values are means of three independent experiments ± SD.