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Electropherogram of a single 100 nm vesicle (1 attoliter in volume) containing Oregon Green (OG) and OG labeled glutamate (OG-Glu) after single-pulse photolysis. The data shows resolved glutamate and free dye peaks. The inset above shows a bulk electropherogram of the glutamate labeling products prior to encapsulation; the images to the right shows the vesicle prior to (top image) and after (bottom image) photolysis; the targeted vesicle is indicated with an arrow.
