Skip to main content
. Author manuscript; available in PMC: 2009 Dec 23.
Published in final edited form as: ACS Nano. 2008 Dec 23;2(12):2519–2525. doi: 10.1021/nn800577h

Figure 1.

Figure 1

Overlaid size exclusion profiles for 3DH-GLE and 3DH-GLE with 1 equiv of added Bis-MTX-C9. Samples were eluted at a flow rate of 0.5 mL/min on Superdex G200 column in P500 buffer monitored by an in-line multiangle static light scattering detector a refractive index detector and a UV detector. (A) 3DH-GLE (20 µM, 500 µL, black) was eluted at 27 min and estimated molecular mass is 64.2 kDa consistent with linear dimeric DHFR-hHint1 as shown in (B); assembly of 3DH-GLE (20 µM) with Bis-MTX-C9 (20 µM, 1 equiv) was shown in red trace and appearance of various sizes of oligomeric rings (16~24 min) and a stable intramolecular macrocyle as shown in (C) was observed.