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. Author manuscript; available in PMC: 2009 Dec 23.
Published in final edited form as: ACS Nano. 2008 Dec 23;2(12):2519–2525. doi: 10.1021/nn800577h

Table 3.

Comparison of steady-state kinetic parameters of hydrolysis of TpAd by 3DHGLE and 3DH-GLE-C9 nanorings isolated from SEC fraction 5, and 6 shown in table 2.

Fraction Protein Oligomer
(Subunit)		 kcat
(s−1)		 Km
(nM)		 kcat/Km
(s−1 M−1)×10−7
NA 3DH-GLE*
Linear monomer		 1 0.73±0.01 28 ± 1 2.6 ± 0.2
5 3DH-GLE-C9
(24.8~27.2 min)
Dimeric Nanoring		 2 0.73±0.01 44 ± 4 1.7 ± 0.2
6 3DH-GLE-C9
(31~32.3 min)
Intramolecular Macrocyle		 1 0.39±0.003 19 ± 1 2.1 ± 0.2
•

Data for 3DH-GLE were adapted from table 1.