Fig. 5.

Immunolabelling of adjacent sections for Calretinin (A–D) and Jag1 (E–H) shows that Cwe/Cwe animals lack most hair cells in the inner ear. (A, B) Calretinin-positive hair cells have developed in the utricle and cristae of wildtype animals at E16.5. In addition, calretinin marks the nuclei in the vestibular ganglion (C–D) Most sections through the Cwe/Cwe inner ear were devoid of hair cells but in a few sections very few calretinin-positive cells are found. The vestibular ganglion marked by calretinin expression appears normal. (E, F) At E16.5 wildtype inner ears have Jag1-positive sensory patches. (G, H) Cwe/Cwe animals have decreased Jag1 labelling. Scale bar = 200 μm. ac, anterior crista; lc, lateral crista; ut, utricle; vg, vestibular ganglion.