Figure 5. Homology model of profalcipain-2.

(a) Model created using MODELLER 9v4. The 160 N-terminal residues of the prodomain are not included in the model. The prodomain (cyan) runs up the face of the mature enzyme (purple; catalytic triad residues in orange) before forming α-helices containing the conserved ERFNIN and GNFD motifs (yellow). (b) Close-up of several predicted interactions between the mature protease and the ERFNIN (R¹⁸⁵) and GNFD (E²¹⁰; F²¹⁴) motifs. Blue dashed lines indicate presumed stabilizing interactions (both electrostatic and hydrophobic) between residues. The structure has been rotated 180 degrees around the vertical axis from its representation in figure 5a.