FIG. 4.

Taconic cohort. Partial pancreatectomy (PP) induced β-cell replication in mice at 2 and 19 months of age. BrdU was administered for 2 weeks after the procedure before the mice were killed. A: Representative pancreatic β-cell histology of pancreas sections immunostained with antibodies against insulin (red) and BrdU (green) and counterstained with DAPI (blue) and photographed with a 40× objective. White arrows indicate insulin and BrdU copositive cells; yellow arrows denote BrdU-labeled non–insulin-containing cells within the islet. Scale bars: 100 μm in full image and 20 μm within inset. B: Quantitative analysis of β-cell regeneration following partial pancreatectomy as a function of age in mice. Results are expressed as percent BrdU-positive β-cells per day and represent means ± SEM (n = 4–6 animals per group). **P < 0.01 sham vs. partial pancreatectomy at 2 months. P <0.05 shams at 2 months vs. shams at 19 months. (A high-quality digital representation of this figure is available in the online issue.)