TABLE 2.
β-Cell morphometry
Age (months) |
|||||
---|---|---|---|---|---|
2 | 8 | 12 | 14 | 19 | |
Pancreatic weight (mg) | |||||
Sham operated (control) | 253 ± 13 | 301 ± 19 | ND | 331 ± 6 | 386 ± 24 |
Partial pancreatectomy | |||||
Resected (splenic) | 127 ± 5 | 169 ± 15 | ND | 184 ± 3 | 187 ± 9 |
Remaining (duodenal) | 108 ± 32 | 113 ± 18 | ND | 204 ± 6 | 212 ± 27 |
β-Cell area (%) | |||||
Sham operated (control) | 0.61 ± 0.09 | 0.85 ± 0.17 | ND | 1.27 ± 0.28 | 2.04 ± 0.36 |
Partial pancreatectomy | |||||
Resected (splenic) | 0.72 ± 0.11 | 1.05 ± 0.13 | ND | 1.90 ± 0.61 | 2.60 ± 0.61 |
Remaining (duodenal) | 0.60 ± 0.04 | 0.79 ± 0.12 | ND | 1.28 ± 0.20 | 2.07 ± 0.55 |
β-Cell mass (mg) | |||||
Sham operated (control) | |||||
Whole pancreas | 1.57 ± 0.26 | 2.46 ± 0.41 | ND | 4.15 ± 0.89 | 7.89 ± 1.59 |
Partial pancreatectomy | |||||
Resected (splenic) | 0.98 ± 0.14 | 1.81 ± 0.31 | ND | 3.48 ± 1.12 | 4.76 ± 1.07 |
Remaining (duodenal) | 0.78 ± 0.23 | 0.93 ± 0.23 | ND | 2.59 ± 0.36 | 4.72 ± 1.75 |
Acinar proliferation (% BrdU/total) | |||||
Sham operated (control) | 0.72 ± 0.08 | 0.57 ± 0.07 | ND | 0.41 ± 0.07 | 0.53 ± 0.11 |
Partial pancreatectomy | 1.47 ± 0.28 | 1.64 ± 0.14 | ND | 1.62 ± 0.24 | 1.07 ± 0.1 |
P | 0.04 | 0.0002 | ND | 0.001 | 0.02 |
Data are means ± SEM for four to seven mice per group except the 19-month-old partial pancreatectomy cohort, which consisted of only three mice. Data show pancreatic weight of sham-operated, resected, or remaining pancreas following partial pancreatectomy, expressed as milligrams; β-cell area of sham-operated, resected (splenic portion), or remaining pancreas (duodenal portion) following partial pancreatectomy, expressed as percent total pancreas; β-cell mass of sham-operated, resected, or remaining pancreas following partial pancreatectomy, expressed as percent total pancreas; and acinar proliferation of sham-operated, resected, or remaining pancreas following partial pancreatectomy, expressed as percent total pancreas. ND, not determined.