TABLE 4.
Relative gene expression of novel candidate diabetic nephropathy susceptibility genes in primary human cell lines
| Cell line | CPVL Ct values | CHN2 Ct values | FRMD3 Ct values | CARS Ct values |
|---|---|---|---|---|
| Endothelial cells, iliac | 17.3 ± 0.7 | 12.5 ± 0.1 | 12.5 ± 0.3 | 8.4 ± 0.4 |
| Fibroblasts | 15.7 ± 0.1 | n.e. | 11.9 ± 0.7 | 6.5 ± 0.4 |
| Mesangial cells | n.e. | n.e. | 12.9 ± 0.5 | 7.2 ± 0.1 |
| Proximal tubules | 8.6 ± 0.1 | 13.1 ± 0.1 | 10.2 ± 0.2 | 8.0 ± 0.1 |
The relative gene expression of CPVL, CHN2, FRMD3, and CARS was determined in four primary human cell lines using real-time PCR. Relative gene expression of each gene was calculated in reference to a normalization control (β-actin) and is presented as the mean ΔCt (Ctvalue from each gene minus Ct value from the normalization control) and SD from three separate experiments. Mean ΔCt = 0 equals high relative gene expression (i.e., expression similar to β-actin). n.e., not expressed (mean ΔCt >40.0).