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. 2009 Mar 16;58(6):1275–1282. doi: 10.2337/db08-1001

FIG. 6.

FIG. 6.

Cytokine and adipokine profiling in TZD-treated Foxo1+/− mice. A and B: Average size of adipocytes from epididymal fat (wild type n = 4; Foxo1+/− n = 5). Representative photomicrographs are represented. Scale bars represent 100 μm. C–E. Multiplexed bead immunoassay techniques were used to measure circulating cytokine and adipokine levels in HFD-fed animals in the presence or absence of TZD. Proinflammatory serum cytokines (tumor necrosis factor-α [TNF-α], IL-6, and monocyte chemoattractant protein 1 [MCP-1]) are significantly reduced in TZD-treated Foxo1+/− mice. Although serum adiponectin levels are lower in HFD-fed Foxo1+/− compared with HFD-fed wild-type, FoxO1 haploinsufficiency is associated with higher elevations in serum adiponectin after TZD treatment. In contrast, serum leptin values decrease to a greater extent in TZD-treated Foxo1+/− animals. These serum cytokine and adipokine data are also presented in numeric form in Table 1. Values are reported as means ± SE. *P < 0.05. ■, HFD wild type; □, HFD Foxo1+/−; ▩, HFD + TZD wild type; ▨, HFD + TZD Foxo1+/−.