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. 2009 Mar 26;58(6):1283–1291. doi: 10.2337/db08-1510

FIG. 3.

FIG. 3.

A siRNA targeting PTPN2 inhibits basal and cytokine-induced PTPN2 expression in INS-1E cells. INS-1E cells were left untransfected (NT □) or transfected with 30 nmol/l of either a control siRNA (siCtrl, ▨) or a pool of siRNAs targeting PTPN2 (siPTPN2, ■). After 2 days of recovery, cells were left untreated, or treated for 24 h with IL-1β (100 units/ml), IFN-γ (100 units/ml), TNF-α (1,000 units/ml), IL-1β (10 units/ml) + IFN-γ (100 units/ml), or TNF-α (1,000 units/ml) + IFN-γ (100 units/ml). A: PTPN2 mRNA expression was assayed by RT-PCR and normalized for the housekeeping gene GAPDH. Results are means ± SE of four independent experiments. B: PTPN2 and α-tubulin protein expression were evaluated by Western blot. The results are representative of five independent experiments. C: Mean optical density measurements of PTPN2 Western blots, corrected for protein loading by α-tubulin. Results are means ± SE of five independent experiments; a: P < 0.001, b: P < 0.01, and c: P < 0.05 vs. untreated NT or untreated transfected with the same siRNA; d: P < 0.01 vs. untreated NT and siCtrl; e: P < 0.01 vs. IL-1β–treated NT and siCtrl; f: P < 0.01 vs. IFN-γ–treated NT and siCtrl; g: P < 0.01 vs. TNF-α–treated NT and siCtrl; h: P < 0.001 vs. IL-1β + INF-γ–treated NT and siCtrl, i: P < 0.01 vs. TNF-α + INF-γ–treated NT and siCtrl; ANOVA followed by Student's t test with Bonferroni correction.