. Author manuscript; available in PMC: 2010 Feb 1.

Published in final edited form as: J Cardiovasc Pharmacol. 2009 Feb;53(2):121–131. doi: 10.1097/FJC.0b013e31819715c4

Table. Comparison of Experimental Approaches to Estimate Cell Numbers.

The table contains summary (normalized to DMSO vehicle controls) data for BAY 41-2272-treated VSM cell numbers after 48 or 72 hours' incubation derived from three different experimental approaches: fluorescent nucleic acid staining, automated cell counting, and hemacytometry. At these time points, BAY significantly reduces VSM cell numbers in dose-dependent fashion

	48 hours			72 hours
	BAY 41-2272 (mM)			BAY 41-2272 (mM)
	0.001	0.1	10.0	0.001	0.1	10.0
Fluorescent nucleic acid staining	93.5 ± 15.5	92.6 ± 12.7	72.4 ± 9.1	60.6 ± 9.5	47.5 ± 1.2	38.5 ±6.3
Automated cell counting	88.2 ± 15.4	77.2 ± 21.9	59.1 ± 11.1	54.9 ± 12.3	41.6 ± 6.3	35.9 ± 12.2
Hemacytometry	79.4 ± 5.6	69.9 ± 11.2	55.0 ± 8.5	61.5 ± 8.8	46.2 ± 8.9	28.6 ± 4.0