Fig. 1.

Stability of capped siRNA and siRNA conjugates in 90% mouse serum at 37 °C. Capped siRNA (B) and siRNA conjugates (C) were incubated with 90% mouse serum at 37 °C at various time intervals and then separated on a 20% PAGE gel with siRNA products detected using a SYBR Gold nucleic acid stain. Lanes A, B and C refer to untreated controls of Dh3 antisense, capped siRNA, and corresponding conjugate, respectively. Lanes 1–10 = 1 min, 2.5 min, 5 min, 15 min, 30 min, 1 h, 3 h, 6 h, 12 h, 24 h serum incubation time. Arrows refer to clip sites as determined in Fig. 2, 3′ inverse deoxythymidine is denoted by idT.