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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Clin Cancer Res. 2009 Mar 24;15(8):2723–2730. doi: 10.1158/1078-0432.CCR-08-1898

Figure 5. HGF drives the acquisition of an epithelial phenotype in subconfluent M15 cells.

Figure 5

M15 cells grown in confluent and subconfluent conditions were treated with 100ng/ml recombinant HGF and stained for vimentin (green) and cytokeratin (red) by immunofluorescence. Nuclei were counterstained with TOPRO-3. An upregulation of cytokeratin was observed after 1 hour in the subconfluent cultures, demonstrating a distinct epithelial filament expression pattern at 24 hours.