Figure 4.
Combining whole-cell clamping with antibody labelling to the proteoglycan NG2 and the nuclear transcription factor Olig2, and nuclear labelling with DAPI. a, Lucifer yellow fill (green) of a cell in a rat cerebellar slice which showed the morphology of an oligodendrocyte precursor. b, Post-recording labelling for NG2 (blue, Lucifer filled cell is arrowed). c, Post recording labelling for Olig2 (red). d, Post-recording labelling for DAPI (white). e, Overlay of a-d showing the cell expresses NG2 and Olig2 and the nucleus is labelled by DAPI. Scale bar 20um.