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. 2009 Apr 3;16(1):38. doi: 10.1186/1423-0127-16-38

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Copyright © 2009 Gireesh et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Representative graph showing displacement analysis of [³H]QNB binding against atropine in the hypothalamus of Control, Diabetic and Diabetic+Insulin treated group rats. Control (black circle), Diabetic (upward pointing black triangle), Insulin treated diabetic rats (downward pointing black triangle). Competition studies were carried out with 1 nM [³H]QNB in each tube with atropine concentrations varying from 10^-9, 10^-4M. Data were fitted with iterative nonlinear regression software (Prism, GraphPad, San Diego, CA). K_iThe affinity of the receptor for the competing drug. EC₅₀is the concentration of the competitor that competes for half the specific binding.