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Plasmid map and synthesis of telomeric RNA transcripts. A, a map of the linearized plasmid containing 96 TTAGGG repeats and the cleavage sites used to generate G-rich (T7 RNA polymerase) and C-rich (T3 RNA polymerase) transcripts. B, electrophoresis of the C-rich RNA (lane 2) and G-rich RNA (lane 3) on an 8 m urea/4% polyacrylamide gel (see “Experimental Procedures”). Lane 1 shows makers of 0.4 kbases and higher.
