FIGURE 2.

Expression patterns of cotransporters and regulatory kinases. A, expression of NKCC1, KCC1, KCC3, KCC4, OSR1, SPAK, WNK1, WNK2, WNK4, in 50B11 cells, and in rat and mouse DRG neurons were demonstrated by RT-PCR. Absence of WNK3 in 50B11 cells and in rat DRG neurons, compared with mouse DRG neurons is due to the lack of rat-specific WNK3 sequence information. B, central nervous system-specific KCC2 was absent in mouse and rat DRG neurons, as well as in the 50B11 cells, but was present in our positive control brain sample.