TABLE 2.

Substrate specificity of DTD3

Initial rates of hydrolysis by purified Synechocystis DTD3 protein (0.2–40 nm) were measured at 28 °C for 5 min in the presence of 20 mm Tris-HCl (pH 7.8), 100 nm of the substrate under study, 3 mm MgCl₂, 40 μm CoCl₂, and 50 μg/ml bovine serum albumin. Values shown are the mean of two independent experiments. Ranges are within 15%.

Substrate	Initial rates
	s-1
d-Tyr-tRNATyr	0.81
l-Tyr-tRNATyr	8.4 × 10-3
Diacetyl-l-Lys-tRNALys	<0.9 × 10-3