TABLE 3.

Comparative analysis between in vitro and in vivo substrates and products of EP24.15

Protein name	Sequence	HEK peptidome in vitroa	Exp. in cellsb	Synthetic peptide in vitroc
Activated RNA polymerase II transcript ion cofactor 4	KEQISDIDDAVRKL	Not substrate	Not substrate
Cathepsin C	DPFNPFELTNH	Substrate	Not substrate
Cytochrome c oxidase subunit VIb	Ac-AEDMETKIKNY	Substrate	Not substrate
Eukaryotic translation elongation factor 1β2	GFGDLKSPAGLQVL	Substrate	Not substrate
Eukaryotic translation initiation factor 4H	VGNLPFNTV	Substrate	Not substrate
Eukaryotic translation initiation factor 5A	AVAIKAMAK		Substrate	Substrate
Eukaryotic translation initiation factor 5A	SAMTEEAAVAIK	Product	Product	Product
Eukaryotic translation initiation factor 5A	MTEEAAVAIKAMAK	Substrate	Substrate
Eukaryotic translation initiation factor 5A	SAMTEEAAVAIKAMAK	Substrate	Substrate	Substrate
FK506-binding protein	VFDVELLKLE	Substrate	Substrate	Substrate
Peptidylprolyl isomerase A	KHTGPGILSM	Substrate	Not substrate
Peptidylprolyl isomerase A	DIAVDGEPLGRVSF	Substrate	Not substrate
Peptidylprolyl isomerase A	ELFADKVPKTAENFR	Substrate	Substrate	Substrate
Ribosomal protein S21	KADGIVSKNF	Substrate	Substrate
Ribosomal protein S21	AKADGIVSKNF	Substrate	Substrate
S100 calcium-binding protein A11	HDSFLKAVPSQKRT		Substrate	Substrate
Splicing factor, arginine/serine-rich 1	AIRDIDLKNR		Substrate	Substrate
Splicing factor, arginine/serine-rich 1	GAIRDIDLKNR	Substrate	Substrate	Substrate
Splicing factor, arginine/serine-rich 1	RGGPPFAFVEF		Substrate	Substrate

^aSelected results from the experiment testing HEK293 cell peptide extracts with 2600 ng of purified EP24.15 are shown (Experiment 1, runs 3 and 4).

^bSelected results from the experiment testing overexpression of EP24.15 in HEK293 cells are shown (Experiment 2, runs 1–4).

^cResults from the experiment testing synthetic peptides with purified EP24.15.