FIGURE 2.

st expression in serum-starved NHF induces CDC6 expression and loading, which is enhanced by coexpression of cyclin E. The kinetics of phosphorylation of CDK2 on Thr-160 and CDK2 substrates is distinct in the soluble and chromatin-bound fraction. BJ-hTERT fibroblasts were serum-starved for 72 h and transduced with indicated adenoviruses (A) or re-stimulated with serum (A and B) and harvested at indicated time points. Soluble and chromatin-bound proteins were obtained and simultaneously analyzed by Western blot. Same exposure times are shown for both fractions. Coomassie Blue staining is shown as a loading control. Relevant proteins are indicated. The CDK2 band corresponding to phosphorylated Thr-160 is indicated with an asterisk.