Expression profiles of the ERRγ in osteoblast progenitor
cells. A, expression of ERRγ mRNA in osteoblast progenitor
cells. Primary osteoblasts, MC3T3-E1, and C2C12 cells were cultured in the
absence or presence of BMP2 (200 ng/ml) for 24 h. RT-PCR was carried out with
the indicated primers. B, expression of ERRγ during osteoblast
differentiation by BMP2. C2C12 cells were cultured in osteogenic medium
containing ascorbic acid (50 μg/ml) and β-glycerophosphate (5
mm) in the presence of BMP2 (200 ng/ml) for 5 days. At the
designated time points, cells were harvested for total RNA isolation, and
RT-PCR was performed with the indicated primers. C and D,
Western blot analysis of ERRγ expression. Primary osteoblasts and C2C12
cells were cultured in osteogenic medium with BMP2 for 5 days. At the
indicated time points, the protein extracts were used for Western blot
analysis with the indicated antibody. E and F,
transactivation of ERRγ promoter by BMP2. C2C12 and MC3T3-E1 cells were
transfected with 200 ng of ERRγ-Luc reporter plasmid and 100 ng of
pCMV-β-galactosidase as an internal control with the indicated amounts of
BMP2, respectively. Data are expressed as the mean ± S.D. of three
independent experiments.