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ERRγ represses Runx2 transcriptional activity. A–F, C2C12 and MC3T3-E1 cells were cotransfected with 200 ng of the indicated luciferase reporter constructs and 100 ng of pcDNA3/Runx2 constructs together with 100 ng of pcDNA3/HA-ERRγ. 48 h after transfection, cells were lysed, and luciferase activity was measured and normalized to β-galactosidase activity. Data are expressed as the mean ± S.D. of three independent experiments.
