FIGURE 2.

Determination of the histatin 3-binding domain of HSC70. A, schematic representation of HSC70 and its mutants. HSC70(Δ1–384), HSC70(Δ385–543), and HSC70(Δ542–646) indicate HSC70 with deleted ATPase, peptide (substrate)-binding, and variable (lid) domains, respectively. All cDNAs were inserted into the pCAT7-neo vector. B, GST pulldown assays for histatin 3 and various HSC70s. Expression vectors encoding various HSC70s were transfected into COS-7 cells, and binding experiments and Western blotting (WB) were carried out as described in Fig. 1E. WT, wild type.