FIGURE 5.

Effect of knockdown of HSC70 in HGFs on DNA synthesis induced by histatin 3. A, HGFs were infected with retroviruses expressing siRNAs targeting HSC70 and its control (cont.). Proteins in cell extracts from HGFs were analyzed by Western blotting with anti-HSC70 (upper) and anti-β-actin (lower) antibodies. B and C, DNA synthesis and cell viability after the addition of histatin 3 to HSC70 knockdown cells were examined as described under “Experimental Procedures.” Bars represent the means of duplicated samples with error bars showing standard deviations. ^*, p < 0.01 (Student's t test).