FIGURE 6.

Effect of heat shock on the localization of histatin 3 and HSC70 in living cells and on DNA synthesis and cell survival. A, HGFs were cultured with FITC-histatin 3 for 12 h and heat-shocked at 42 °C for 5 h (right) or not treated (left). Cells were stained with anti-HSC70 antibody and 4′,6-diamidino-2-phenylindol dihydrochloride (DAPI) (nuclear staining), and images were obtained using confocal laser microscopy. Upper panel, localization of HSC70; middle panel, localizations of histatin 3 and HSC70; lower panel, merged images of histatin 3 and HSC70. B and C, DNA synthesis and cell viability in the presence of histatin 3 under heat shock conditions were examined as described under “Experimental Procedures.” Bars represent the means of duplicated samples with error bars showing standard deviations. ^*, p < 0.01 (Student's t test). cont. pep., control peptide.