FIGURE 7.
Effect of 15-DSG on DNA synthesis, cell survival, and the binding of HSC70 to p27Kip1 after treatment of cells with histatin 3. A and B, DNA synthesis and cell viability in the presence of 15-DSG and histatin 3 were examined as described under “Experimental Procedures.” Bars represent the means of duplicated samples with error bars showing standard deviations. *, p < 0.01 (Student's t test). C, effect of 15-DSG on the binding of HSC70 to p27Kip1 after the addition of histatin 3 to cells. HGFs were cultured in DMEM containing 0.1% FBS over 24 h and stimulated with serum, P3a, and histatin 3 in the presence or absence of 15-DSG. At 24 h, proteins extracted from cells were immunoprecipitated (IP) with anti-p27Kip1 antibody. Precipitates were analyzed by Western blotting (WB) with anti-HSC70 (top) and anti-p27Kip1 (bottom) antibodies.