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. 2009 May 22;284(21):14396–14404. doi: 10.1074/jbc.M808116200

FIGURE 1.

FIGURE 1.

Loss of ATM impairs proliferation in astrocytes. A, astrocytes were isolated from the brains of newborn Atm+/+ and Atm-/- mice, and their growth curves were determined by trypan blue staining at indicated days after initial seeding of cells. The means ± S.D. of three independent experiments are shown. *, p < 0.05 when Atm-/- astrocytes were compared with Atm+/+ astrocytes. B, phase contrast photomicrographs of Atm+/+ and Atm-/- astrocytes following further incubation for 4 days (left panel). ATM in C1 astrocytes was inactivated by ATM inhibitor KU55933, and knocked down using ATM siRNA (right panel). Scale bars, 50 μm. C, the proportions of senescent cells in Atm+/+ and Atm-/- astrocytes were determined by SA β-galactosidase expression 2 days after cultivation. The means ± S.D. of three independent experiments are shown. *, p < 0.05 when Atm-/- astrocytes were compared with Atm+/+ astrocytes.