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. 2009 May 22;284(21):14396–14404. doi: 10.1074/jbc.M808116200

FIGURE 4.

FIGURE 4.

In Atm-/- astrocytes, ERK1/2 is constitutively activated and CDK inhibitors are up-regulated. A and B, for Atm+/+ and Atm-/- astrocytes, levels of phospho-ERK1/2, ERK1/2, and Bmi-1 (A) and levels of p53, p21cip1, and p16Ink4a (B) were assessed by direct Western blotting analysis. C, levels of Bmi-1, p53, p21cip1, p16Ink4a, and phospho-Rb were compared for Atm+/+ and Atm-/- cerebella tissues by direct Western blotting analysis. The means ± S.D. of three different mice tissues are shown. *, p < 0.05; **, p < 0.01 when Atm-/- tissues was compared with Atm+/+ tissues. D, Atm+/+ and Atm-/- astrocytes were treated with 100 μm H2O2. The cells were then harvested to access levels of Bmi-1 and p16Ink4a at the indicated times after H2O2 treatment. E, Atm+/+ and Atm-/- astrocytes were either left untreated or treated with 100 μm H2O2. Levels of phospho-ERK1/2, ERK1/2, p16Ink4a, and phospho-Rb were determined by direct Western blotting analysis.