FIGURE 5.

Glutamate and pyruvate stimulate the succinate supported H₂O₂ generation only in the non-BSA-brain mitochondria. Incubation conditions were as in Fig. 1. Final volume was 1 ml. Additions were as follows: 5 μm Amplex red, 3 units of horseradish peroxidase, 50 units of superoxide dismutase (Sigma), 0.1 mg of rat brain mitochondria, 5 mm succinate (S), 5 mm glutamate (G), 2.5 mm pyruvate (P), 2 mm malate (M). Malonate (5 mm) was added before BM. Numbers associated with traces show the rates of H₂O₂ production in pmol of H₂O₂/min/mg of BM protein. AU, arbitrary units.