TABLE 1.
Dissociation constants of α8β1 integrin toward nephronectin and its deletion mutants
| Ligands | Kda |
|---|---|
| nm | |
| NN | 0.28 ± 0.01 |
| RGD-linker | 0.23 ± 0.05 |
| MAM | ND (–)b |
| RGD-linker (GST) | 0.22 ± 0.04 |
| PRGDV (GST) | NDc |
| LS/366–414 | 0.32 ± 0.03 |
| LS/378–414 | 0.33 ± 0.02 |
| LS/366–393 | ND |
| LS/378–407 | 0.33 ± 0.01 |
| LS/378–403 | 0.44 ± 0.04 |
| LS/378–401 | 7.1 ± 0.6 |
| LS/378–399 | 13 ± 1 |
| LS/378–393 | ND |
| LS/381–407 | 0.42 ± 0.08 |
| LS/391–407 | ND |
| LS/395–407 | ND |
a
Values represent the mean ± S.D. of three independent experiments
b
ND, not determined. The dissociation constant was not determined owing to the absence of significant binding
c
The dissociation constants were not determined owing to only partial saturation at the highest integrin concentration examined