TABLE 2.
Dissociation constants of α8β1 integrin toward LS/378 – 403 after alanine scanning mutagenesis
| Ligands | Kda |
|---|---|
| nm | |
| LS/378–403 | 0.44 ± 0.04 |
| L395A | 0.44 ± 0.08 |
| F396A | 0.84 ± 0.22 |
| E397A | 0.42 ± 0.06 |
| I398A | 0.38 ± 0.04 |
| F399A | 0.72 ± 0.12 |
| E400A | 0.95 ± 0.12 |
| I401A | 1.3 ± 0.2 |
| E402A | 2.5 ± 0.4 |
| R403A | 0.51 ± 0.08 |
| E397/400A | 0.81 ± 0.23 |
| E397/402A | 4.4 ± 0.6 |
| E400/402A | NDb |
a
Values represent the mean ± S.D. of three independent experiments
b
ND, not determined. The dissociation constant was not determined due to only partial saturation at the highest integrin concentration examined