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Calnexin mRNA is detectable during late stages of CD34+ differentiation. A, reverse transcription-PCR analysis of the expression pattern of calnexin and AE1 mRNA in differentiating primary erythroid progenitors. As an RNA control, the 18S rRNA gene was also amplified by reverse transcription-PCR. B, relative RNA expression of calnexin and AE1 to 18S rRNA during erythroid differentiation. WT, wild type; IP, immunoprecipitation.
