TABLE 1.
Protease
|
Photoadduct
massa
|
Assignment
|
Ref.
|
||
---|---|---|---|---|---|
Glycosylated | Deglycosylated | ||||
kDa | |||||
Chymotrypsin | 50 (47) | 31 | L1/part of CR domainb | 6 | |
Chymotrypsin | 34 | 23 | C-terminal fragmentc | 34 | |
Chymotrypsin | 20 | 17 | ID-Nd | 34 | |
Trypsin | 31 (28) | 20 (17) | L1e | 6 |
Molecular masses in parentheses are exclusive of the respective A- or B-chain adduct.
The fragment contains ∼250-260 residues (supplemental Fig. S2).
The fragment contains (from the C to N terminus) ID-N, Fn2a, and part of Fn1.
The fragment is likely to begin within Fn2a.
The fragment is likely to contain initial residues of the CR domain (supplemental Fig. S1).