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. 2009 May 22;284(21):14597–14608. doi: 10.1074/jbc.M900087200

TABLE 1.

Key receptor fragments in photomapping strategy

Protease
Photoadduct massa
Assignment
Ref.
Glycosylated Deglycosylated
kDa
Chymotrypsin 50 (47) 31 L1/part of CR domainb 6
Chymotrypsin 34 23 C-terminal fragmentc 34
Chymotrypsin 20 17 ID-Nd 34
Trypsin 31 (28) 20 (17) L1e 6
a

Molecular masses in parentheses are exclusive of the respective A- or B-chain adduct.

b

The fragment contains ∼250-260 residues (supplemental Fig. S2).

c

The fragment contains (from the C to N terminus) ID-N, Fn2a, and part of Fn1.

d

The fragment is likely to begin within Fn2a.

e

The fragment is likely to contain initial residues of the CR domain (supplemental Fig. S1).