FIGURE 4.

Gemin7 is important for formation of the subcore complex. A, Gemin7 gene silencing with siRNA. Cytoplasmic extracts were prepared from untreated (WT), Gemin7 siRNA transfected (siGemin7), and negative control siRNA transfected (control siRNA) HeLa cells. The extracts were analyzed by Western blotting using anti-Gemin7 antibody. B, cytoplasmic soluble fraction from the siRNA (siGemin7)-treated and untreated (WT) HeLa cells were immunoprecipitated with anti-SMN antibody. Five percent of the input (left panel) and immunoprecipitates (IP) (right panel) was analyzed by Western blotting using anti-SMN, Gemin2, SmB/B′, and SmE antibodies.