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. 2009 May 22;284(21):14645–14656. doi: 10.1074/jbc.M807631200

FIGURE 4.

FIGURE 4.

Oligomerization analysis and hypothetical dimer configurations. A, chromatograms of ALO from S200 size exclusion chromatography coupled to differential pressure transducers and SLS and UV detectors. Data lines show UV (solid), RALS (dot-dash), LALS (dot-dot-dash), and DP (dotted). B, molecular weight (MW) and intrinsic viscosity (IV) patterns of ALO. Data lines show molecular weight (solid), LALS (dot-dot-dash), and IV (dotted). The retention volume of ALO (22.2 ml) is probably due to interactions with the column media. Using standard proteins for calibration, such a retention volume corresponds to a 15-20-kDa protein (not shown). Hence, measurement of molecular mass by SEC-SLS, which is independent from the retention volume on SEC, is appropriate for ALO. C, sedimentation velocity analysis of ALO from analytical ultracentrifugation at three different pH values: pH 5.5 (dashed), pH 7.0 (solid), and pH 8.0 (dotted). D, potential dimer interactions. Crystallographic dimers are found in ALO and PFO (I) crystals, and noncrystallographic dimers are seen in PFO (III) and ILY crystals. Domains 1-3 are colored blue, and D4 is colored orange.