RT-PCR product encoding LAT1 was detected in cDNA from one (I) embryo equivalent at timed stages of development 1C, 1-cell; 2C, 2-cell; 4C, 4-cell; 8C, 8-cell; M, morula; B, blastocyst; HB, hatching blastocyst (A). Representative image of three independent replicates. Quantitative analysis of LAT1 are shown in (B). mRNA transcript levels in a developmental series of mouse preimplantation embryos by Real-Time RT-PCR. Data were normalized to external Luciferase control (0.5pg/embryo) and relative to 4-cell target gene mRNA levels. Relative mRNA levels are presented as the mean ± s.e, representative of three independent replicates. Bars with different letters represent significant differences in relative mRNA levels between embryo stages (p≤0.05).