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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Biochem Pharmacol. 2008 Nov 25;77(6):1074–1083. doi: 10.1016/j.bcp.2008.11.013

Fig. 2.

Fig. 2

Selective toxicity of anti-RLIP76 IgG, RLIP76 siRNA and RLIP76 antisense toward PC-3 prostate cancer cells Effect of anti-RLIP76 IgG (40 μg/ml final concentration) on cell survival was determined by MTT assay. Depletion of RLIP76 expression by RLIP76 siRNA (20 μg/ml final conc.) and RLIP76 antisense (10 μg/ml final conc.) was done, using Transmessenger Transfection-Reagent-kit (Qiagen), and Maxfect Transfection-Reagent (Molecula, Inc.), according to the manufacturer’s instructions, respectively. Cell survival was measured by MTT cytotoxicity-assay 48 h after treatment [18]. The values are presented as mean ± SD from two separate determinations with eight-replicates each (n = 16). black bars, HUVEC (normal) cells; gray bars, PC-3 prostate cancer cells.