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. 2009 Apr 28;106(19):7786–7791. doi: 10.1073/pnas.0811750106

Fig. 3.

Fig. 3.

Full time-course analysis of the kinetics of ProT activation by VWbp(1-263). (A) Progress curves for hydrolysis of d-Phe-Pip-Arg-pNA by mixtures of VWbp(1-263) and ProT, at 200 μM substrate (black points), contained 1 nM ProT and 0.025, 0.075, 0.15, 0.3, 0.6, 3, or 10 μM VWbp. Substrate depletion assays (red points) contained 1 nM ProT, 1 μM VWbp, and concentrations of 7, 20, 40, 78, or 116 μM substrate. (B) Progress curves for hydrolysis of 200 μM Tosyl-Gly-Pro-Arg-pNA (black points) contained 1 nM ProT and 0.1, 0.3, 0.5, 0.75, 1, 3, or 10 μM VWbp. Substrate depletion assays (red points) contained 1 nM ProT, 5 μM VWbp, and 25, 50, 75, 100, 150, or 500 μM substrate. Representative examples of the whole dataset are shown for each substrate, which contained 17–24 progress curves over 0.025–10 μM VWbp and 5–500 μM substrate. Data are shown as every tenth point, and the fit with the mechanism in Scheme 1 and parameters in Table 1 is represented by solid lines.