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. 2009 Apr 3;106(19):7708–7713. doi: 10.1073/pnas.0811918106

Fig. 2.

Fig. 2.

DANS-based comparison of daf-22 and wild-type metabolomes. (A) Schematic representation of DANS. C. elegans wild-type metabolite extract has dauer-inducing and male-attracting activity, whereas daf-22 metabolite extract is inactive (8, 19). Comparison of wild-type and daf-22 metabolomes via DANS reveals candidate molecules for biological evaluation (red). (B) Aliphatic section of the dqfCOSY spectrum of C. elegans wild-type metabolite extract prior to differential analysis, revealing the complexity of this extract. (C) DANS overlay of the wild-type spectrum in Fig. 2B with the corresponding region of the daf-22 spectrum. Signals present in both wild-type and daf-22 spectra cancel or change color (blue), whereas signals representing compounds only present in wild type remain unaffected (brown), most prominently signals representing part of the side chains of ascr#2 and ascr#3.