Fig. 5.

Deletion of HDAC1 and HDAC2 during neuronal differentiation induces cell death. (A) Live-staining of neuronal precursors after 24 h in neuronal differentiation media. Scale bar = 5 microns. Control-GFP are propidium iodide (PI)-negative, whereas Cre-GFP cells are PI-positive and show fragmented nuclei indicative of cell death. (B) TUNEL analysis of E15.5 wild-type and mutant mice shows significant lethality in the ventricular zone of mutant mice, whereas no cell death is observed in wild-type littermates. Scale bar: 100 microns.